Body Integrity Identity Disorder (BIID) is a rare and complicated disorder related to the individuals who feel a ORCH infections including Toxoplasmosis, Other infections, Rubella, Cytomegaloviruses and Herpes simplex are major maternal infections that may cause severe fetal outcomes, such as congenital malformations, intrauterine growth retardation, and neurodevelopmental issues in the long-term. Although they have an effect, there are a lot of pregnant women who are not aware about these infections and how they can affect them. Health literacy, which has been described as the capacity to obtain, comprehend, and implement health-related information, is a very important factor in enhancing maternal knowledge and the development of preventive measures. This research was conducted to determine the efficiency of a health literacy program on enhancing knowledge on TORCH infection among antenatal mothers at one of the selected Primary Health Centres (PHC) in Puducherry. One-group pre-test and post-test research design design was used, where 51 antenatal mothers were recruited using the convenience sampling method. The data was collected based on a structured instrument that had demographic information and a standardized knowledge test. The pre and post intervention levels of knowledge were compared and assessed with descriptive and inferential statistics. The results showed that during the pre-test, 92.2% of the participants showed a poor level of knowledge, 7.8% moderately knowledgeable, and 0 showed adequate knowledge. The outcome of the post-intervention revealed an impressive improvement since only 3.9% were still inadequate, 56.9% achieved moderate knowledge, and 39.2% reached an adequate level of knowledge. This increase was found significant (p < 0.001).This research established that health literacy interventions are able to make a significant contribution to maternal knowledge of TORCH infections and thus improve mother and neonatal health outcomes.